Regulation of Single-Strand Annealing and its Role in Genome Maintenance

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• 作者：Ragini Bhargava¹ ; ² ; David O. Onyango¹ ; Jeremy M. Stark¹ ; ² ; ^{jstark@coh.org" class="auth_mail" title="E-mail the corresponding author}
• 关键词：alternative end joining ; end resection ; homology directed repair ; RAD51 ; RAD52 ; single-strand annealing
• 刊名：Trends in Genetics
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：32
• 期：9
• 页码：566-575
• 全文大小：1798 K

文摘

Single-strand annealing (SSA) is a DNA double-strand break (DSB) repair pathway that uses homologous repeats to bridge DSB ends. SSA involving repeats that flank a single DSB causes a deletion rearrangement between the repeats, and hence is relatively mutagenic. Nevertheless, this pathway is conserved, in that SSA events have been found in several organisms. In this review, we describe the mechanism of SSA and its regulation, including the cellular conditions that may favor SSA versus other DSB repair events. We will also evaluate the potential contribution of SSA to cancer-associated genome rearrangements, and to DSB-induced gene targeting.