Microscopy tools for the investigation of intracellular lipid storage and dynamics

详细信息    查看全文

• 作者：Sabine Daemen¹ ; ^{sabine.daemen@maastrichtuniversity.nl" class="auth_mail" title="E-mail the corresponding author} ; Marc A.M.J. van Zandvoort² ; ³ ; ^{mamj.vanzandvoort@maastrichtuniversity.nl" class="auth_mail" title="E-mail the corresponding author} ; Sapun H. Parekh⁴ ; ^{parekh@mpip-mainz.mpg.de" class="auth_mail" title="E-mail the corresponding author} ; Matthijs K.C. Hesselink¹ ; ^{matthijs.hesselink@maastrichtuniversity.nl" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Metabolic disease ; Lipid droplets ; Fluorescent lipid probes ; Super-resolution ; Live-cell imaging ; Vibrational microscopy
• 刊名：Molecular Metabolism
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：5
• 期：3
• 页码：153-163
• 全文大小：2082 K

文摘

Excess storage of lipids in ectopic tissues, such as skeletal muscle, liver, and heart, seems to associate closely with metabolic abnormalities and cardiac disease. Intracellular lipid storage occurs in lipid droplets, which have gained attention as active organelles in cellular metabolism. Recent developments in high-resolution microscopy and microscopic spectroscopy have opened up new avenues to examine the physiology and biochemistry of intracellular lipids.

Scope of review

The aim of this review is to give an overview of recent technical advances in microscopy, and its application for the visualization, identification, and quantification of intracellular lipids, with special focus to lipid droplets. In addition, we attempt to summarize the probes currently available for the visualization of lipids.

Major conclusions

The continuous development of lipid probes in combination with the rapid development of microscopic techniques can provide new insights in the role and dynamics of intracellular lipids. Moreover, in situ identification of intracellular lipids is now possible and promises to add a new dimensionality to analysis of lipid biochemistry, and its relation to (patho)physiology.