One-step reverse transcription loop-mediated isothermal amplification for the detection of Maize chlorotic mottle virus in maize

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• 作者：Ling Chen^a ; Zhiyuan Jiao^a ; Dongmei Liu^a ; Xingliang Liu^b ; Zihao Xia^a ; Congliang Deng^b ; Tao Zhou^a ; Zaifeng Fan^a ; ^{fanzf@cau.edu.cn}
• 关键词：Maize chlorotic mottle virus (MCMV) ; Loop-mediated isothermal amplification (LAMP) ; Maize ; Detection
• 刊名：Journal of Virological Methods
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：240
• 期：Complete
• 页码：49-53
• 全文大小：960 K
• 卷排序：240

文摘

A one-step RT-LAMP was developed for the diagnosis of MCMV in field-grown maize. The RT-LAMP procedure could be completed within 60 min using the primers designed in the CP gene. The RT-LAMP assay was about 10-fold more sensitive than RT-PCR in this study. The RT-LAMP for detecting MCMV is specific and feasible.