A rapid molecular diagnosis of cutaneous leishmaniasis by colorimetric malachite green-loop-mediated isothermal amplification (LAMP) combined with an FTA card as a direct sampling tool
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- 作者：Chukwunonso O. Nzelua ; Abraham G. Cá ; ceresb ; c ; Silvia Guerrero-Quinchod ; Edwin Tineo-Villafuertee ; Luis Rodriquez-Delfinf ; Tatsuyuki Mimorig ; Hiroshi Uezatoh ; Ken Katakuraa ; Eduardo A. Gomezi ; Angel G. Guevaraj ; Yoshihisa Hashiguchij ; k ; l ; Hirotomo Katoa ; hkato@vetmed.hokudai.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Leishmania ; LAMP ; FTA ; Malachite green ; Peru
- 刊名：Acta Tropica
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：153
- 期：Complete
- 页码：116-119
- 全文大小：356 K
文摘
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A LAMP assay for rapid detection of Leishmania DNA from an FTA card was established.
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The colorimetric FTA-LAMP was highly sensitive enough to detect 0.01 parasites.
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The field applicability was demonstrated with clinical samples collected from patients with CL in Peru.
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The FTA-LAMP will be an important tool for diagnosis of cutaneous leishmaniasis.