Performance of a MALDI-TOF MS-based imipenem hydrolysis assay incorporating zinc sulfate

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• 作者：James Knox^a ; ^b ; ^{jaknox2@yahoo.com.au} ; ^{james.knox@mps.com.au} ; Enzo Palombo^a ; ^{epalombo@swin.edu.au}
• 关键词：MALDI-TOF MS ; Zinc ; Carbapenemase ; Metallo-beta-lactamase ; Gram-negative ; Enterobacteriaceae
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：87
• 期：3
• 页码：258-260
• 全文大小：210 K
• 卷排序：87

文摘

Improved testing is a vital part of the global response to carbapenemase-producing Gram-negatives. A MALDI-TOF MS-based imipenem hydrolysis assay was enhanced with the addition of zinc ions. This modification was necessary for the detection of some metallo-β-lactamase producing strains. With this improvement, the assay demonstrated excellent sensitivity and specificity