Synaptic plasticity alterations associated with memory impairment induced by deletion of CB2 cannabinoid receptors

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• 作者：Mar¨ªa S. Garc¨ªa-Guti¨¦rrez ; Antonio Ortega-¨¢lvaro ; Arnau Busquets-Garc¨ªa ; Jose M. P¨¦rez-Ortiz ; Laura Caltana ; Mar¨ªa Jimena Ricatti ; Alicia Brusco ; Rafael Maldonado ; Jorge Manzanares
• 关键词：CB2r ; Mice lacking CB2r ; Memory ; Cannabinoid CB2r-agonist JWH133 ; Immunohistochemistry ; Rt-PCR
• 刊名：Neuropharmacology
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：73
• 期：Complete
• 页码：388-396
• 全文大小：1660 K

文摘

In this study, the role of CB₂r on aversive memory consolidation was further evaluated. Mice lacking CB₂r (CB2KO) and their corresponding littermates (WT) were exposed to the step-down inhibitory avoidance test (SDIA). MAP2, NF200 and synaptophysin (SYN)-immunoreactive fibers were studied in the hippocampus (HIP) of both genotypes. The number of synapses, postsynaptic density thickness and the relation between the synaptic length across the synaptic cleft and the distance between the synaptic ends were evaluated in the HIP (dentate gyrus (DG) and CA1 fields) by electron microscopy. Brain-derived neurotrophic factor (BDNF), glucocorticoid receptor (NR3C1) gene expressions and mTOR/p70S6K signaling cascade were evaluated in the HIP and prefrontal cortex (PFC). Finally, the effects of acute administration of CB₂r-agonist JWH133 or CB₂r-antagonist AM630 on memory consolidation were evaluated in WT mice by using the SDIA.

The lack of CB₂r impaired aversive memory consolidation, reduced MAP2, NF200 and SYN-immunoreactive fibers and also reduced the number of synapses in DG of CB2KO mice. BDNF and NR3C1 gene expression were reduced in the HIP of CB2KO mice. An increase of p-p70S6K (T389 and S424) and p-AKT protein expression was observed in the HIP and PFC of CB2KO mice. Interestingly, administration of AM630 impaired aversive memory consolidation, whereas JWH133 enhanced it. Further functional and molecular assessments would have been helpful to further support our conclusions.

These results revealed that CB₂r are involved in memory consolidation, suggesting that this receptor could be a promising target for developing novel treatments for different cognitive impairment-related disorders.