TLR and NLRP3 inflammasome expression deregulation in macrophages of adult rats subjected to neonatal malnutrition and infected with methicillin-resistant Staphylococcus aureus

详细信息    查看全文

• 作者：Natá ; lia Gomes de Morais ; Ph.D.^a ; ^{Natalia.morais@univasf.edu.br} ; Thacianna Barreto da Costa ; Ph.D.^b ; Joana Maria Bezerra de Lira ; B.A.^a ; Suê ; nia da Cunha Gonç ; alves de Albuquerque^c ; Valé ; ria Rê ; go Alves Pereira ; Ph.D.^d ; Milena de Paiva Cavalcanti ; Ph.D.^e ; Cé ; lia Maria Machado Barbosa de Castro ; Ph.D.^f
• 关键词：Malnutrition ; Macrophage ; Infection ; Inflammasome ; MRSA
• 刊名：Nutrition
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：33
• 期：Complete
• 页码：174-180
• 全文大小：1456 K
• 卷排序：33

文摘

Nutritional aggression in critical periods may lead to epigenetic changes that affect gene expression. The aim of this study was to assess the effect of neonatal malnutrition on the expression of toll-like receptor (TLR)-2, TLR-4, and NLRP3 receptors, caspase-1 enzyme, and interleukin (IL)-1 β production in macrophages infected with methicillin-resistant (MRSA) and methicillin-sensitive (MSSA) Staphylococcus aureus.MethodsWistar rats (N = 24) were divided in two distinct groups: nourished (17% casein) and malnourished (8% casein). Four systems were established after the isolation of mononuclear cells: negative control, positive control, MRSA, and MSSA. The plates were incubated at 37°C for 24 h in humidified atmosphere and 5% carbon dioxide. Tests were performed after this period to analyze the expression of standard recognition receptors, caspase-1 enzyme, and the production of IL-1 β. Student's t test and analysis of variance were used in the statistical analysis; P < 0.05 was statistically significant.ResultsMalnutrition reduced animal growth and the expression of TLR-2, TLR-4, and NLRP3 receptors, the caspase-1 enzyme, and the IL-1 β levels in macrophages infected with lipopolysaccharides in the present study. However, the interaction between the S. aureus and the macrophages promoted greater gene expression of receptors and enzymes.ConclusionThe neonatal malnutrition model compromised the expression of standard recognition receptors, of the caspase-1 enzyme as well as the production of IL-1 β. However, the S. aureus and neonatal malnutrition combination led to intense transcription of such innate immunity components. Therefore, the deregulation in the expression of TLR and NLRP3 receptors and of the caspase-1 enzyme may induce extensive tissue injury and favor the permanence and spread of these bacteria, especially those that are methicillin resistant.