High-yield soluble expression, purification and characterization of human steroidogenic acute regulatory protein (StAR) fused to a cleavable Maltose-Binding Protein (MBP)
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- 作者：Nikolai N. Sluchankoa ; nikolai.sluchanko@mail.ru" class="auth_mail" title="E-mail the corresponding author ; Kristina V. Tugaevaa ; b ; Yaroslav V. Faletrovc ; Dmitrii I. Levitskya ; d
- 关键词：Steroidogenic acute regulatory protein ; Soluble expression ; Maltose-Binding Protein ; Fluorescence ; Steroid hormones ; Cholesterol
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：119
- 期：Complete
- 页码：27-35
- 全文大小：1183 K
文摘
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StAR protein has limited solubility and is mostly obtained through denaturation/refolding.
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This is time-consuming, costly and may affect protein characteristics.
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Fusing cleavable MBP to StAR confers its full solubility and facilitates purification.
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The developed protocol results in native and aggregates-free StAR.
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Capable of binding cholesterol, it is suitable for future investigations and biotechnology.