β-Funaltrexamine inactivates ORL1 receptors in BE(2)-C human neuroblastoma cells

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• 作者：Mandyam ; Chitra D. ; Altememi ; Ghazi F. ; Standifer ; Kelly M.
• 关键词：μ ; -Opioid receptor ; cAMP accumulation ; Orphanin FQ/nociceptin
• 刊名：European Journal of Pharmacology
• 出版年：2000
• 出版时间：August 18, 2000
• 年：2000
• 卷：402
• 期：1-2
• 页码：205-207
• 全文大小：200 K

文摘

The potential interactions of natively expressed μ-opioid and opioid receptor-like (ORL1) receptors were studied by exposing intact BE(2)-C cells to agonists or antagonists for 1 h. Pretreatment with the μ-opioid receptor agonist, [d-Ala²,N-Me-Phe⁴,Gly⁵-ol]enkephalin (DAMGO), or the ORL1 receptor agonist, orphanin FQ/nociceptin desensitized both μ-opioid and ORL1 receptor responses. β-Funaltrexamine (β-FNA) pretreatment also blocked both μ-opioid and ORL1 receptor responses, but only μ-opioid receptor binding was reduced. Moreover, β-FNA (1 μM) failed to inhibit specific ORL1 receptor binding.