Calcium-mediated modulation of Pseudomonas mendocina NR802 biofilm influences the phenanthrene degradation

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• 作者：Neelam Mangwani ; Sudhir K. Shukla ; T. Subba Rao ; Surajit Das
• 关键词：Biofilm ; Degradation ; Extracellular polymeric substances ; Phenanthrene ; Roughness coefficient
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：1 February, 2014
• 年：2014
• 卷：114
• 期：Complete
• 页码：301-309
• 全文大小：1661 K

文摘

A potential biofilm forming and phenanthrene utilizing marine bacterium Pseudomonas mendocina NR802 was isolated from Rushukulya, Odisha, East Coast of India. The effect of Ca²⁺ and Mg²⁺ on biofilm growth and phenanthrene degradation was evaluated. Among the various tested concentrations, 20 mM of Ca²⁺ and Mg²⁺ showed a significant enhancement in biofilm production by the bacterium. The SEM-EDAX study showed that the elemental composition of the biofilm varied significantly when grown in the presence of Ca²⁺ and Mg²⁺. The CSLM analysis of biofilms grown in the presence of 20 mM Ca²⁺ and Mg²⁺ reveal the critical role of these ions on biofilm architectural parameters such as total biomass, biofilm thickness, roughness coefficient and surface to biovolume ratio. Ca²⁺ was found to enhance the extracellular polymeric substances (EPS) production and phenanthrene degradation. Ca²⁺ enhanced the biofilm growth in a dose dependent manner, whereas Mg²⁺ significantly increased the cell growth in biofilm. More than 15% increase in phenanthrene degradation was observed when biofilm was grown in the presence of an additional 20 mM Ca²⁺. This study also supports the fundamental role of Ca²⁺ in biofilm growth, architecture as well as biofilm-mediated pollutant degradation.