A thermotolerant Endo-1,4-β-mannanase from Trichoderma virens UKM1: Cloning, recombinant expression and characterization
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- 作者：Sin Yee Chaia ; sinyeechai23@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Farah Diba Abu Bakarb ; fabyff@ukm.edu.my" class="auth_mail" title="E-mail the corresponding author ; Nor Muhammad Mahadic ; nor.mahadi@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Abdul Munir Abdul Muradd ; munir@ukm.edu.my" class="auth_mail" title="E-mail the corresponding author ; munir8488@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Trichoderma virens ; β-mannanase ; Pichia pastoris ; Recombinant protein ; Thermotolerant
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：125
- 期：Complete
- 页码：49-57
- 全文大小：2196 K
文摘
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An endo-β-mannanase from T. virens UKM1 was successfully cloned and expressed as functionally active enzyme in P. pastoris.
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Expressed recombinant mannanase (rMANTV) showed high optimum temperature at 70 °C.
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It showed 70% of its activity at 80 °C and good stability along 20–65 °C.
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rMANTV showed good thermotolerance with more than 60% of retaining activity at 60 °C up to one hour.
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rMANTV has a half-life of 26 min at 70 °C.