An endo-β-mannanase from T. virens UKM1 was successfully cloned and expressed as functionally active enzyme in P. pastoris.
Expressed recombinant mannanase (rMANTV) showed high optimum temperature at 70 °C.
It showed 70% of its activity at 80 °C and good stability along 20–65 °C.
rMANTV showed good thermotolerance with more than 60% of retaining activity at 60 °C up to one hour.
rMANTV has a half-life of 26 min at 70 °C.