Aneurinifactin, a new lipopeptide biosurfactant produced by a marine Aneurinibacillus aneurinilyticus SBP-11 isolated from Gulf of Mannar: Purification, characterization and its biological evaluation

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• 作者：Shanmugasundaram Senthil Balan^a ; ^c ; C. Ganesh Kumar^b ; ^{cgkumar@iict.res.in} ; ^{cgkumar5@gmail.com} ; Singaram Jayalakshmi^a
• 关键词：Aneurinibacillus aneurinilyticus ; Lipopeptide ; Biosurfactant ; Emulsification ; Antimicrobial ; Oil recovery
• 刊名：Microbiological Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：194
• 期：Complete
• 页码：1-9
• 全文大小：1109 K
• 卷排序：194

文摘

Biosurfactants are microbial-derived amphiphilic molecules having hydrophobic and hydrophilic moieties produced by bacteria, fungi, yeasts and algae and are extracellular or cell wall-associated compounds. In an ongoing survey for bioactive microbial metabolites from microbes isolated from diverse ecological niches, a new lipopeptide biosurfactant was identified from a marine bacterium; Aneurinibacillus aneurinilyticus strain SBP-11, which was isolated from a marine diversity hotspot, Gulf of Mannar, India. A new lipopeptide biosurfactant was purified and characterized based on TLC, FT-IR, NMR, GC–MS, HPLC, MALDI-TOF-MS and tandem MS analysis as Stearic acid-Thr-Tyr-Val-Ser-Tyr-Thr (named as Aneurinifactin). The critical micelle concentration of Aneurinifactin was 26 mg L−1 at a surface tension of 26 mN m−1. Further, the biosurfactant showed stable emulsification at a wide range of pH (2–9) and temperature up to 80 °C. Aneurinifactin showed promising antimicrobial activity and concentration dependent efficient oil recovery. This is the first report on Aneurinifactin, a lipopeptide biosurfactant produced by a marine A. aneurinilyticus SBP-11, which could be explored as a promising candidate for use in various biomedical and industrial applications.