- 作者：Jaime Gos¨¢lvez ; Carlos Garc¨ªa-Ochoa ; Miguel Ru¨ªz-Jorro ; Manuel Mart¨ªnez-Moya ; Pascual S¨¢nchez-Mart¨ªn ; Pedro Caballero
- 关键词：Espermatozoide ; Factor masculino ; Criopreservació ; n ; Fragmentació ; n del á ; cido desoxirribonucleico ; Donante de semen
- 刊名：Revista Internacional de Androlog¨ªa
- 出版年：2013
- 出版时间：July-September, 2013
- 年：2013
- 卷：11
- 期：3
- 页码：85-93
- 全文大小：731 K
Objective
The study was made to analyze the baseline levels of damage recorded in sperm DNA fragmentation (SDF) and to estimate sperm DNA longevity as observed in donors after thawing.
Material and methods
Fifty donors and forty individuals attending a clinic and classified as a normo-zoospermic population were compared. The baseline SDF levels and the increasing rate of SDF (r-SDF) obtained after thawing when the sperm was incubated for a period of 24 h with different sub-sampling performed after 2, 6 and 24 h of incubation were considered as the independent variables and compared.
Results
Cryopreserved donor sperm exhibited baseline SDF values approximately 2 times lower than those observed in the control group. DNA stability was 2.5 times higher than that observed in the control cohort. Baseline values of SDF of approximately 8 % generates 65 % sensitivity and 82 % specificity to discriminate between the donors and controls. Values of increase of damage of 1.8 % per hour, analyzed during the first hours of incubation, identify the donor characteristics with 77 % sensibility and 65 % specificity. Neither value show any correlation within the control and donor cohorts group.
Conclusion
The establishment of these types of threshold values can be used to identify donors considered as ¡°super-donors¡± in relation to their low levels of SDF and high chromatin stability. The donors selected from the different clinics participating in this study showed similar characteristics for these parameters.