Growth pattern switch of renal cells and expression of cell cycle related proteins at the early stage of diabetic nephropathy

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• 作者：Yanling Zhang ; Yonghong Shi ; Yaling Liu ; Hui Dong ; Maodong Liu ; Ying Li ; Huijun Duan
• 关键词：Diabetic nephropathy ; Proliferation ; Hypertrophy ; Cyclin E ; CDK₂ ; P²⁷
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2007
• 出版时间：9 November 2007
• 年：2007
• 卷：363
• 期：1
• 页码：159-164
• 全文大小：401 K

文摘

Renal hypertrophy, partly due to cell proliferation and hypertrophy, has been found correlated to renal function deterioration in diabetes mellitus. We screened the up-regulated cell cycle related genes to investigate cell growth and the expression of cell cycle regulating proteins at the early stage of diabetic nephropathy using STZ-induced diabetic rats. Cyclin E, CDK₂ and P²⁷ were found significantly up-regulated in diabetic kidney. Increased cell proliferation in the kidney was seen at day 3, peaked at day 5, and returned to normal level at day 30. Cyclin E and CDK₂ expression also peeked at day 5 and P²⁷ activity peaked at day 14. These findings indicate that a hyperplastic growth period of renal cells is followed by a hypertrophic growth period at the early stage of diabetes. The growth pattern switch may be regulated by cell cycle regulating proteins, Cyclin E, CDK₂, and P²⁷.