- 作者：AgataRadwanska ; Dagmara Baczynska ; Dorota Nowak ; Sté ; phane Bré ; zillon ; Agnieszka Popow ; Franç ; ois-Xavier Maquart ; Yanusz Wegrowski ; Maria Malicka-Blaszkiewicz
- 关键词：Extracellular matrix ; Lumican ; Actin cytoskeleton ; Actin isoforms ; A375 melanoma cells
- 刊名：Life Sciences
- 出版年：2008
- 出版时间：7 November 2008
- 年：2008
- 卷：83
- 期：19-20
- 页码：651-660
- 全文大小：1263 K
AimsLumican, a small leucine-rich proteoglycan (SLRP), has attracted attention as a molecule of the extracellular matrix possibly involved in signalling pathways affecting cancer cell behaviour. The remodelling of the actin cytoskeleton, induced in response to external stimuli, is crucial for cell motility and intracellular signal transduction. The main goal of this study was to examine the effects of recombinant lumican on actin organization, the state of actin polymerization, actin isoform expression, and their sub-cellular distribution in the A375 human melanoma cell line.Main methods
Fluorescence and confocal microscopy were used to observe actin cytoskeletal organization and the sub-cellular distribution of cytoplasmic β- and γ-actins. The ability of actin to inhibit DNaseI activity was used to quantify actin. Western blotting and real-time PCR were used to determine the expression levels of the actin isoforms.
Key findings
A375 cells grown on lumican coatings changed in morphology and presented rearranged actin filament organization: from filaments evenly spread throughout the whole cell body to their condensed sub-membrane localization. In the presence of lumican, both actin isoforms were concentrated under the cellular membrane. A statistically significant increase in the total, filamentous, and monomeric actin pools was observed in A375 cells grown on lumican.
Significance
Novel biological effects of lumican, an extracellular matrix SLRP, on the actin pool and organization are identified, which may extend our understanding of the mechanism underlying the inhibitory effect of lumican on the migration of melanoma cells.