Production of soybean phosphatidylcholine–chitosan nanovesicles by reverse phase evaporation: a step by step study
详细信息 查看全文
- 作者：Omar Mertins ; Marcelo Sebben ; Adriana Raffin Pohlmann and Ná ; dya Pesce da Silveira
- 关键词：Soybean phosphatidylcholine ; Liposomes ; Nanovesicles ; Reverse micelles ; Chitosan
- 刊名：Chemistry and Physics of Lipids
- 出版年：2005
- 出版时间：2005
- 年：2005
- 卷：138
- 期：1-2
- 页码：29-37
- 全文大小：276 K
文摘
In the present work, we describe the preparation of composite nanovesicles containing soybean phosphatidylcholine and polysaccharide chitosan by the reverse phase evaporation method. Nanovesicles free from chitosan prepared in the same way were studied as reference. The production method involves the preparation of reverse micelles followed by the formation of an organogel, which is dispersed in water to yield the final liposomal structures. Structural changes in each step of the nanovesicles preparation were studied by means of static and dynamic light scattering as well as small angle X-ray scattering. Chitosan was also fully characterized in solution. The hydrodynamic radius of the composite nanovesicles is in the range of 174–286 nm, depending on the chitosan contents. A comparison with nanovesicles free from chitosan indicates the existence of higher contents of multilamellae structures in the composites, as well as improved stability in water.