Simple method for purification of enterotoxigenic Escherichia coli fimbriae
详细信息 查看全文
- 作者：Brittany Curtisa ; b ; Christen Grassela ; b ; Rachel S. Laufera ; b ; Khandra T. Searsa ; c ; Marcela F. Pasettia ; c ; Eileen M. Barrya ; b ; Raphael Simona ; b ; rsimon@medicine.umaryland.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Purification ; Fimbriae ; Escherichia coli
- 刊名：Protein Expression and Purification
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：119
- 期：Complete
- 页码：130-135
- 全文大小：809 K
文摘
- •
ETEC fimbriae expressed from plasmids in Escherichia coli production strains.
- •
Orthogonal purification steps: size, solubility, charge.
- •
Anion-exchange membranes used to capture fimbriae.
- •
Fimbriae highly purified, recognized by convalescent human sera.