Serum levels of beta-2-microglobulin-free heavy chain of HLA class I antigen in healthy individuals: relationship to their class I allotype
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- 作者：Perosa ; Federico ; Prete ; Marcella ; Luccarelli ; Grazia ; Favoino ; Biagio ; Ferrone ; Soldano ; Dammacco ; Franco
- 关键词：Ag ; antigen(s) ; β ; 2m ; beta-2-microglobulin ; BSA ; bovine serum albumin ; DDIA ; double determinant immunoassay ; HLA-I ; human major histocompatibility class I antigen ; mAb ; monoclonal antibody ; PBS ; phosphate buffered saline ; SDS-PAGE ; sodium dodecyl sulf
- 刊名：Human Immunology
- 出版年：1999
- 出版时间：November, 1999
- 年：1999
- 卷：60
- 期：11
- 页码：1058-1066
- 全文大小：424 K
文摘
An ELISA-based double determinant immunoassay has been established to measure the soluble β2-microglobulin (β2m)-free heavy chain (FHC) of the HLA-B, -C (and HLA-A3, -A28 and -A30) class I molecular complex in sera from 212 HLA-typed healthy unrelated individuals. FHC was calculated by means of a standard curve constructed using serial concentrations of β2m-associated HLA-class I heavy chain (HLA-I)/FHC purified from cultured human lymphoid cell C1R-sB7-supernatant. The mean FHC concentration (±SD) was 0.25mg/l (±0.2). Its median concentration did not statistically differ between males and females, though the male/female ratio was greater in the high secretor (FHC >0.45 mg/l; mean+1SD) than in the low secretor group (FHC < 0.05mg/l; mean-1SD). FHC < 0.05mg/l was statistically (Fisher’s exact test) associated with HLA-B17 (p = 0.003); FHC > 0.45mg/l was statistically associated with HLA-B35 (p = 0.003) and −Cw4 (p = 0.002). None of these allele-positive groups showed a mean FHC concentration 1.5 times higher than that of the corresponding allele-negative ones. This allotype-dependent HLA-B and C FHC enhancement was less marked than that previously reported for HLA-I in individuals carrying HLA-A9 (and its splits). These results indicate that FHC could be a more valuable marker when its levels are compared among individuals carrying different allotypes. Moreover the lack of correlation between FHC and HLA-I levels measured in 52 HLA-A3, -A28 or -A30 positive individuals suggests that the two molecules may be regulated by different metabolic pathways and their serum expression may have a different biological significance.