Structural Insights into the HWE Histidine Kinase Family: The Brucella Blue Light-Activated Histidine Kinase Domain
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- 作者：Jimena Rinaldi1 ; Mehrnoosh Arrar2 ; Gabriela Sycz1 ; Marí ; a Laura Cerutti1 ; 3 ; Paula M. Berguer1 ; &dagger ; ; Gastó ; n Paris1 ; Darí ; o Ariel Estrí ; n2 ; Marcelo Adriá ; n Martí ; 4 ; Sebastiá ; n Klinke1 ; 3 ; Fernando Alberto Goldbaum1 ; 3 ; fgoldbaum@leloir.org.ar" class="auth_mail" title="E-mail the corresponding author
- 关键词：LOV-HK ; blue light-activated histidine kinase ; HK ; histidine kinase ; SHK ; sensor histidine kinase ; LOV ; blue-light sensor ; SEC ; size-exclusion chromatography ; SLS ; static light &ndash ; scattering ; MD ; molecular dynamics
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：27 March 2016
- 年：2016
- 卷：428
- 期：6
- 页码：1165-1179
- 全文大小：2188 K
文摘
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The crystal structure of the Brucella HK domain reveals two distinct dimers.
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In solution, the Brucella HK domain is an active monomer.
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The canonical dimer is the functionally relevant species.
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The autophosphorylation occurs in cis.
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A catalytic mechanism for Brucella LOV-HK is proposed.