Human fibroblast culture on a crosslinked dermal porcine collagen matrix

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• 作者：Jarman-Smith ; Marcus ; Bodamyali ; Tulin ; Stevens ; Cliff ; Howell ; John A. ; Horrocks ; Michael ; et. al.
• 关键词：Biomedical ; Bioreactors ; Collagen ; Dermis ; Fibroblasts tissue cell culture
• 刊名：Biochemical Engineering Journal
• 出版年：2004
• 出版时间：August 15, 2004
• 年：2004
• 卷：20
• 期：2-3
• 页码：217-222
• 全文大小：279 K

文摘

The use of a novel porcine-derived collagen biomaterial as a dermal tissue engineering matrix was examined. The matrix is derived from porcine dermis, and is processed to retain the native collagen (Type 1) and elastin structure. Human primary fibroblasts were cultured on the matrix to examine its potential for creating a dermal replacement. Attachment of fibroblasts on the collagen was compared to tissue culture plastic and PET membranes. Cell proliferation was assessed using the MTT assay and DAPI staining. For seeding densities of 5×10⁴ and 1×10⁵cellscm⁻², PET and plastic demonstrated >95 % attachment of seeded numbers after 3h. The collagen matrix reached levels >80 % after 3–4h with no influence of the seeding density. Matrix samples with perforating pores of 40μm diameter were also studied. After 216h culture in static culture, with media replacement every 3 days, the final cell numbers reached 2.1×10⁵ (perforated) and 2.0×10⁵cellscm⁻² (unperforated). In comparison fibroblast culture in a perfusion bioreactor, with continuous media replacement, reached 2.3×10⁵ (unperforated) and 2.5×10⁵cellscm⁻² (perforated) after 216h.