Enterobacteria and Vibrio from Macrobrachium amazonicum prawn farming in Fortaleza, Ceará, Brazil

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• 作者：D&eacute ; bora de Souza Collares Maia Castelo-Branco¹ ; Jamille Alencar Sales² ; Raimunda Sâ ; mia Nogueira Brilhante¹ ; ^{brilhante@ufc.br" class="auth_mail" title="E-mail the corresponding author} ; Glaucia Morgana de Melo Guedes¹ ; Yago Brito de Ponte² ; C&eacute ; lia Maria de Souza Sampaio² ; Tereza de Jesus Pinheiro Gomes Bandeira¹ ; Jos&eacute ; Luciano Bezerra Moreira¹ ; Lucas Pereira de Alencar² ; Manoel de Ara&uacute ; jo Neto Paiva² ; Rossana de Aguiar Cordeiro¹ ; Andr&eacute ; Jalles Monteiro³ ; Waldemiro de Aquino Pereira-Neto¹ ; Jos&eacute ; J&uacute ; lio Costa Sidrim¹ ; Marcos F&aacute ; bio Gadelha Rocha¹ ; ²
• 关键词：Prawn ; Water ; Enterobacteria ; Vibrio ; Antibiotics
• 刊名：Asian Pacific Journal of Tropical Medicine
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：9
• 期：1
• 页码：27-31
• 全文大小：304 K

文摘

To investigate the isolation of enterobacteria associated with <em>Macrobrachium amazonicumem> (<em>M. amazonicumem>) farming and evaluate the <em>in vitroem> antimicrobial susceptibility of <em>Vibrioem> strains.

ec_2">Methods

Strains were isolated from female <em>M. amazonicumem> prawns and environmental and hatchery water. Biochemical assays were used to identify bacterial genera and those belonging to the genus <em>Vibrioem> were submitted to further analyses for species identification, through Vitek 2 automated system and serotyping. Susceptibility test was performed according to Clinical Laboratory Standards Institute.

ec_3">Results

The following genera of enterobacteria were recovered: <em>Enterobacterem> (<em>nem> = 11), <em>Citrobacterem> (<em>nem> = 10), <em>Proteusem> (<em>nem> = 2), <em>Serratiaem> (<em>nem> = 2), <em>Kluyveraem> (<em>nem> = 2), <em>Providenciaem> (<em>nem> = 2), <em>Cedeceaem> (<em>nem> = 1), <em>Escherichiaem> (<em>nem> = 1), <em>Edwardsiellaem> (<em>nem> = 1) and <em>Buttiauxellaem> (<em>nem> = 1). As for <em>Vibrioem>, three species were identified: <em>Vibrio choleraeem> non-O1/non-O139 (<em>nem> = 4), <em>Vibrio vulnificus (V. vulnificus)em> (<em>nem> = 1) and <em>Vibrio mimicusem> (<em>nem> = 1). <em>Vibrioem> spp. showed minimum inhibitory concentrations values within the susceptibility range established by Clinical Laboratory Standards Institute for almost all antibiotics, except for <em>V. vulnificusem>, which presented intermediate profile to ampicillin.

ec_4">Conclusions

Enterobacteria do not seem to be the most important pathogens associated with <em>M. amazonicumem> farming, whereas the recovery of <em>Vibrioem> spp. from larviculture, with emphasis on <em>Vibrio choleraeem> and <em>V. vulnificusem>, deserves special attention due to their role as potentially zoonotic aquaculture-associated pathogens. Furthermore, the intermediate susceptibility of <em>V. vulnificusem> to ampicillin reflects the importance of monitoring drug use in prawn farming.