Further insight into the pH effect on the catalysis of mushroom tyrosinase
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- 作者：Miguel Angel Maria-Solano<sup>asup> ; Carmen Vanessa Ortiz-Ruiz<sup>asup> ; Jose Luis Muñ ; oz-Muñ ; oz<sup>asup> ; Jose Antonio Teruel-Puche<sup>bsup> ; Jose Berna<sup>csup> ; Pedro Antonio Garcia-Ruiz<sup>dsup> ; Francisco Garcia-Canovas<sup>asup><sup> ; sup><sup>canovasf@um.es" class="auth_mail" title="E-mail the corresponding authorsup>
- 关键词：Em ; met-tyrosinase ; Ed ; deoxy-tyrosinase ; Eox ; oxy-tyrosinase ; EmD ; met-tyrosinase/o-diphenol complex ; EoxD ; oxy-tyrosinase/o-diphenol complex ; EmM ; met-tyrosinase/monophenol complex ; EoxM ; oxy-tyrosinase/monophenol complex ; EmH ; met-tyrosinase/proton complex ; EoxH ; oxy-tyrosinase/proton complex ; D ; o-diphenol ; [D]ss ; o-diphenol concentration in steady-state ; M ; monophenol ; [M]ss ; monophenol concentration in steady-state ; R ; ratio between [D]ss and [M]ss ; R=[D]ss/[M]ss &asymp ; [D]ss/[M]0 ; Q ; o-q
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：125
- 期：Complete
- 页码：6-15
- 全文大小：1418 K
文摘
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Protons behave as competitive-type inhibitors of tyrosinase.
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Tyrosinase pK<sub>asub> causes a sigmoidal behavior in the dependence of initial rate vs. pH.
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E322 could be responsible for the pH effect acting as a gatekeeper.
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A bell-shaped dependence occurs if the pK<sub>asub> of the phenolic hydroxyl groups are low.
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Negatively charged substrates in the R group cooperate in the bell-shaped behavior.