Association of cytokine gene polymorphisms and serum concentrations with the outcome of chronic hepatitis B

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• 作者：Simone R.S. Conde^a ; ^b ; Rosimar N.M. Feitosa^a ; Felipe Bonfim Freitas^a ; Renata B. Hermes^a ; Samia Demachki^b ; Marialva T.F. Araú ; jo^c ; Manoel C.P. Soares^c ; Ricardo Ishak^a ; Antonio C.R. Vallinoto^a ; ^{vallinoto@ufpa.br}
• 关键词：Chronic hepatitis B ; Cytokine gene polymorphisms ; Cytokine serum levels
• 刊名：Cytokine
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：61
• 期：3
• 页码：940-944
• 全文大小：298 K

文摘

Objective

The present paper investigated possible correlations between the clinical presentation of hepatitis B and the TNF-¦Á ?308G/A, IFN-¦Ã +874A/T, TGF-beta1 ?509C/T, and IL-10 ?1081A/G polymorphisms and associated serum levels of these cytokines.

Methods

Fifty-three hepatitis patients were selected and divided into two groups: A - inactive (n = 30) and B - chronic hepatitis/cirrhosis (n = 23). The control group consisted of 100 subjects who were positive for anti-HBc and anti-HBs. The serum concentrations of the cytokines were determined by immunoenzymatic assays. The polymorphisms of the cytokines genes were assessed by PCR and PCR-SSP.

Results

The mean serum levels of IFN-¦Ã of the control group were significantly higher than those of groups A and B, whereas the mean levels TGF-beta1 were significantly higher in groups A and B in comparison with the control. In the case of IL-10, the mean serum level recorded in the control group was significantly higher than that of group B. The TNF-¦Á ?308AG genotype was considerably more frequent in group B (43.3 % ) than the control (14.4 % ).

Conclusion

Higher serum levels of IFN-¦Ã and TGF-beta1 were associated with chronic hepatitis B, and lower serum levels of IL-10 were found in patients with the active disease. Furthermore the presence of allele A of the TNF-¦Á ?308 polymorphism suggest a risk of the progressive disease.