Cellular immune response evaluation of cutaneous leishmaniasis patients cells stimulated with Leishmania (Viannia) braziliensis antigenic fractions before and after clinical cure
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- 作者：Maria Carolina Accioly Brelaz-de-Castro ; carolina.brelaz@gmail.com ; Amanda Ferreira de Almeida ; Andresa Pereira de Oliveira ; Marina de Assis-Souza ; Lucas Ferreira da Rocha ; Valé ; ria Rê ; go Alves Pereira
- 关键词：American cutaneous leishmaniasis ; Immune response ; T lymphocyte ; Antigen ; Flow cytometry ; Cytokines
- 刊名：Cellular Immunology
- 出版年：2012
- 出版时间：October, 2012
- 年：2012
- 卷：279
- 期：2
- 页码：180-186
- 全文大小：734 K
文摘
American cutaneous leishmaniasis (ACL) is a disease where susceptibility or resistance is dependent on T cell response. This is characterized by an increased in CD4+ T cells, capable of inducing opposite disease profiles, and CD8+ T cells, that are related to immuno protection. We characterized T lymphocytes from patients before and after treatment, patients that spontaneously healed and controls, also evaluating their production of IL-10, IL-4, TNF-¦Á and IFN-¦Ã, after stimulation with soluble/insoluble antigenic fractions of Leishmania (Viannia) braziliensis. We observed the production of suppressive cytokines in the early phase of leishmaniasis with significant presence CD4+ T cells, suggesting their connection with disease progression. After healing, the immune pattern observed was a type 1 response, what seems to be associated with cure and/or protection in the ACL. The results also showed that both fractions induced a specific immune response, contributing to the search for relevant antigens in this disease.