GC/MS analysis revealed thymol as a major component of T. algeriensis, T. vulgaris and T. serpyllum, with its contribution to the oil 56.0%, 48.9% and 38.5%, respectively. All three essential oils (EOs) exhibited a significant antimicrobial activity against all tested strains, the T. serpyllum oil being the most potent one (MIC 2.5-5 渭g/mL, MBC 5-10 渭g/mL; MIC 1-2 渭g/mL, MFC 2-4 渭g/mL). In addition, the T. serpyllum oil revealed the highest antioxidant activity in all conducted assays and it was the most effective one against all tested cell lines, presenting GI50 values of 7.02-52.69 渭g/mL. Moreover, none of the EOs showed toxicity at tested concentrations (>400 渭g/mL) for porcine liver primary cell culture. In addition to their well-known traditional use in food and cosmetics, the great potential of tested Thymus essential oils for application in oral diseases and anticancer treatments, encourage further investigation.