We demonstrate effective growth cone remodelling using a 5 % criterion (near-scanning).
Recurrent line near-scanning aligned growth cone processes along the scan line.
The new membrane protrusions, marked by DiI, started a few minutes after scanning onset and progressively grew in thickness.
Using scanning patterns suitable for connecting distinct growth cones, new links were consistently developed.
Although the underlying mechanism is still a matter for investigation, a mechanical perturbation produced by the moving probe appeared to induce the process formation. Thanks to its deterministic and interactive features, this novel approach to guiding growth cones is a promising way to develop networks of identified neurons as well as link them with artificial structures.