Use of scanning ion conductance microscopy to guide and redirect neuronal growth cones

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• 作者：Mario Pellegrino ; Paolo Orsini ; Francesca De Gregorio
• 关键词：Growth cone ; Guidance ; Scanning ion conductance microscopy ; Leech
• 刊名：Neuroscience Research
• 出版年：2009
• 出版时间：July 2009
• 年：2009
• 卷：64
• 期：3
• 页码：290-296
• 全文大小：479 K

文摘

Scanning ion conductance microscopy has been applied to neuronal growth cones of the leech either to image or to stimulate them. Growth cone advance was recorded in non-contact mode using a 2 % ion current decrease criterion for pipette-membrane distance control.

We demonstrate effective growth cone remodelling using a 5 % criterion (near-scanning).

Recurrent line near-scanning aligned growth cone processes along the scan line.

The new membrane protrusions, marked by DiI, started a few minutes after scanning onset and progressively grew in thickness.

Using scanning patterns suitable for connecting distinct growth cones, new links were consistently developed.

Although the underlying mechanism is still a matter for investigation, a mechanical perturbation produced by the moving probe appeared to induce the process formation. Thanks to its deterministic and interactive features, this novel approach to guiding growth cones is a promising way to develop networks of identified neurons as well as link them with artificial structures.