Versatile bio-ink for covalent immobilization of chimeric avidin on sol–gel substrates

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• 作者：Jarkko J. Heikkinen^a ; ^{jarkko.j.heikkinen@oulu.fi"" rel=""nofollow} ; Liisa Kivim&#xe4 ; ki^b ; ^{liisa.kivimaki@vtt.fi"" rel=""nofollow} ; Juha A.E. M&#xe4 ; &#xe4 ; tt&#xe4 ; ^c ; ^{juha.maatta@uta.fi"" rel=""nofollow} ; Inka M&#xe4 ; kel&#xe4 ; ^b ; ^{inka.makela@vtt.fi"" rel=""nofollow} ; Leena Hakalahti^b ; ^{leena.hakalahti@vtt.fi"" rel=""nofollow} ; Kristiina Takkinen^d ; ^{kristiina.takkinen@vtt.fi"" rel=""nofollow} ; Markku S. Kulomaa^c ; ^{markku.kulomaa@uta.fi"" rel=""nofollow} ; Vesa P. Hytö ; nen^c ; ^{vesa.hytonen@uta.fi"" rel=""nofollow} ; Osmo E.O. Hormi^a ; ^{osmo.hormi@oulu.fi"" rel=""nofollow}
• 关键词：Biomolecule immobilization ; Printing ; Sol– ; gel ; Maleimide ; Chimeric avidin ; Avidin– ; biotin technology ; Biosensing
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：2011
• 出版时间：15 October 2011
• 年：2011
• 卷：87
• 期：2
• 页码：409-414
• 全文大小：912 K

文摘

A bio-ink for covalent deposition of thermostable, high affinity biotin-binding chimeric avidin onto sol–gel substrates was developed. The bio-ink was prepared from heterobifunctional crosslinker 6-maleimidohexanoic acid N-hydroxysuccinimide which was first reacted either with 3-aminopropyltriethoxysilane or 3-aminopropyldimethylethoxysilane to form silane linkers 6-maleimide-N-(3-(triethoxysilyl)propyl)hexanamide or -(ethoxydimethylsilyl)propyl)-hexanamide. C-terminal cysteine genetically engineered to chimeric avidin was reacted with the maleimide group of silane linker in methanol/PBS solution to form a suspension, which was printed on sol–gel modified PMMA film. Different concentrations of chimeric avidin and ratios between silane linkers were tested to find the best properties for the bio-ink to enable gravure or inkjet printing. Bio-ink prepared from 3-aminopropyltriethoxysilane was found to provide the highest amount of active immobilized chimeric avidin. The developed bio-ink was shown to be valuable for automated fabrication of avidin-functionalized polymer films.