Immunohistochemistry of apoptosis-related proteins in retinoblastoma

详细信息    查看全文

• 作者：Renato José ; Mendonç ; a Natalino ; MDp>ap>p> ; p>p>renato.natalino@hc.fm.usp.brp>p> ; p>p>renato.j.m.natalino@gmail.comp> ; Cé ; lia Beatriz Gianotti Antoneli ; PhDp>bp> ; Karina de Cá ; ssia Braga Ribeiro ; PhDp>cp> ; Antô ; nio Hugo José ; Fró ; es Marques Campos ; PhDp>ap> ; Fernando Augusto Soares ; PhDp>ap>
• 关键词：pRb ; retinoblastoma protein ; AI ; apoptotic index ; IAP ; inhibitors of apoptosis proteins
• 刊名：Pathology - Research and Practice
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：212
• 期：12
• 页码：1144-1150
• 全文大小：2254 K
• 卷排序：212

文摘

Retinoblastoma is the most common intraocular malignant neoplasia during childhood and results from the partial or total inactivity of the retinoblastoma protein (pRb). In the absence of pRb, the E2F transcription factors increase the levels of cell cycle proteins as well as some pro-apoptotic proteins. We intended to study the immunohistochemistry profile of apoptotic-related proteins in retinoblastoma. We also evaluated the association between the expression of apoptotic protein and stage of tumor or survivor after a 5 year follow up. Apoptosis-related proteins (Apaf-1, Bak, Bax, Bcl-2, Bcl-xL, Bim-long, MDM2, p53, pro-caspase-3, PUMA, Smac/DIABLO and cleaved caspase-3) were evaluated using immunohistochemistry on tissue microarrays which contained samples of retinoblastoma tumors taken from ninety-three patients without any treatment previous to surgery. The immunohistochemistry reactions were evaluated using an optical microscope as well as the ACIS III® platform. The pro-apoptotic proteins (APAF-1, Bax, p53, PUMA, Smac/DIABLO) were more frequently expressed than the anti-apoptotic proteins (Bcl-2, Bcl-xL and MDM2). The protein Bcl-xL had a negative correlation with cleaved caspase-3, a marker of cell apoptosis. Bcl-xL may be implicated in an apoptosis block.