- 作者：Fern ; a Rossatto Temp ; Aline Carr茅 Santos ; Joseane Righes Marafiga ; Ana Cl谩udia Jesse ; Qu茅li Fern ; es Lenz ; Sara Marquesan Oliveira ; Gustavo Petri Guerra ; Teresa Nieves Scimonelli ; Carlos Fern ; o Mello
- 关键词：Melanocyte-stimulating-hormone ; Central inflammation ; Epilepsy
- 刊名：Life Sciences
- 出版年：6 November, 2013
- 年：2013
- 卷：93
- 期：20
- 页码：723-731
- 全文大小：1011 K
Aims
Alpha-melanocyte stimulating hormone (伪-MSH) is a pro-opiomelanocortin (POMC)-derived peptide involved in different neurological functions that also exerts anti-inflammatory effects, including in the central nervous system (CNS). Although inflammation has been implicated in seizures and epilepsy, no study has systematically investigated whether 伪-MSH modifies seizures. Therefore, in the current study we determined whether 伪-MSH alters pentylenetetrazol (PTZ)- and pilocarpine-induced seizures.Main methods
Adult male Swiss mice were injected with 伪-MSH (1.66, 5 or 15 渭g/3 渭L, intracerebroventricular (i.c.v.)) or systemic (0.1, 0.3 or 1 mg/kg, intraperitoneally (i.p.)). Five to sixty minutes after the injection of the peptide, animals were injected with PTZ (60 mg/kg, i.p.) or pilocarpine (370 mg/kg, i.p.). Latency to myoclonic jerks and tonic-clonic seizures, number of seizure episodes, total time spent seizing and seizure intensity, assessed by the Racine and Meurs scales were recorded. Interleukin 1 beta (IL-1尾) levels in the hippocampus were measured by a commercial enzyme-linked immunoabsorbent assay (ELISA).
Key findings
Neither intracerebroventricular (1.66, 5 or 15 渭g/3 渭L, i.c.v.) nor systemic (0.1, 0.3 or 1 mg/kg, i.p.) administration of 伪-MSH altered PTZ- and pilocarpine-induced seizures. IL-1尾 levels in the hippocampi were not altered by 伪-MSH, PTZ or pilocarpine.
Significance
Although inflammation has been implicated in seizures and epilepsy and 伪-MSH is a potent anti-inflammatory peptide, our results do not support a role for 伪-MSH in seizure control.