Molecularly imprinted polymer solid-phase extraction for the analysis of organophosphorus pesticides in fruit samples

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• 作者：Mohd Marsin Sanagi ; Syairah Salleh ; Wan Aini Wan Ibrahim ; Ahmedy Abu Naim ; Dadan Hermawan ; Mazidatulakmam Miskam ; Iqbal Hussain ; Hassan Y. Aboul-Enein
• 关键词：Organophosphorus pesticides (OPPs) ; Molecularly imprinted polymers (MIPs) ; Fruit samples ; Food analysis ; Food composition ; HPLC ; MIP-SPE ; C18-SPE ; NIP-SPE ; Food safety ; Food contamination ; Agricultural practices ; Maximum residue limits in food ; Pesticide in food
• 刊名：Journal of Food Composition and Analysis
• 出版年：December, 2013
• 年：2013
• 卷：32
• 期：2
• 页码：155-161
• 全文大小：872 K

文摘

A new selective material based on molecularly imprinted polymers (MIPs) was prepared and used as solid-phase extraction (SPE) sorbent for sample enrichment of organophosphorus pesticides (OPP) residues prior to high performance liquid chromatography (HPLC). Three OPPs widely used in agriculture (diazinon, quinalphos and chlorpyrifos) were selected as target analytes. Various parameters affecting the extraction efficiency of the imprinted polymers were evaluated to optimize the selective preconcentration of OPPs from water samples. Under the optimized conditions, the developed MIP-SPE method showed excellent linearity in the range of 4-200 渭g L^鈭? with coefficient of determination (r²) > 0.997 and good OPP recoveries of >91% and limits of detection (LODs) ranging from 0.83 渭g L^鈭? to 2.8 渭g L^鈭?,which is much lower than the maximum residue limits (MRLs) set by the Codex Alimentarius Commission and Japan Food Chemical Research Foundation. The developed method was successfully applied to the analysis of OPPs in selected fruit samples. MIP-SPE showed superior extraction efficiency towards the OPPs as compared to non-imprinted polymer solid-phase extraction (NIP-SPE) and commercial C₁₈-SPE methods.