Pre-hydrolysis with carbohydrases facilitates the release of protein from brewer¡¯s spent grain

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• 作者：Piritta Niemi ^{piritta.niemi@vtt.fi} ; Duarte Martins¹ ; ^{duarte.dasilvamartins@gmail.com} ; Johanna Buchert ^{johanna.buchert@vtt.fi} ; Craig B. Faulds ; ^{craig.faulds@univ-amu.fr}
• 关键词：BSG ; Brewer&rsquo ; s spent grain ; MW ; molecular weight ; rBSG-D ; Depol 740 hydrolysis residue ; rBSG-D/AcP ; Depol 740 hydrolysis residue treated with Acid Protease A ; rBSG-D/Alc ; Depol 740 hydrolysis residue treated with Alcalase ; rBSG-D/P144 ; Depol 740 hydr
• 刊名：Bioresource Technology
• 出版年：2013
• 出版时间：May, 2013
• 年：2013
• 卷：136
• 期：Complete
• 页码：529-534
• 全文大小：551 K

文摘

Brewer¡¯s spent grain (BSG) is the most abundant side-stream from brewing. It is food-grade being rich in dietary fibre and protein and thus having potential as their source for both food and non-food applications. Initial treatment of milled BSG with a carbohydrase cocktail from Humicola insolens significantly enhanced the subsequent solubilisation of protein from the residual biomass. When treated with an alkaline protease, 76 % of BSG protein was solubilized, whereas the yields were significantly lower with neutral or acidic proteases. In alkaline conditions significant amount of protein (53 % ) as predominantly low molecular weight protein was solubilized even without any protease addition. The degree of protein solubilisation was influenced by the time of exposure of modified BSG to the alkaline environment. The non-enzymatic protein solubilisation was, however, only observed when BSG had been initially treated with the carbohydrase, suggesting the protein is surrounded by cell wall polysaccharides restricting its initial release.