IC-tagged proteins are able to interact with each other and perform complex reactions when integrated into muNS-derived inclusions

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• 作者：Alberto Brandariz-Nuñ ; ez¹ ; ² ; Iria Otero-Romero¹ ; Javier Benavente ; Jose M. Martinez-Costas ; ^{jose.martinez.costas@usc.es}
• 关键词：Protein&ndash ; protein interactions ; IC-tagging ; Particulate protein inclusions ; Reovirus
• 刊名：Journal of Biotechnology
• 出版年：2011
• 出版时间：20 September, 2011
• 年：2011
• 卷：155
• 期：3
• 页码：284-286
• 全文大小：1K

文摘

We have recently developed a versatile tagging system (IC-tagging) that causes relocation of the tagged proteins to ARV muNS-derived intracellular globular inclusions. In the present study we demonstrate (i) that the IC-tag can be successfully fused either to the amino or carboxyl terminus of the protein to be tagged and (ii) that IC-tagged proteins are able to interact between them and perform complex reactions that require such interactions while integrated into muNS inclusions, increasing the versatility of the IC-tagging system. Also, our studies with the DsRed protein add some light on the structure/function relationship of the evolution of DsRed chromophore.