In silico optimization and low structured kinetic model of poly[(R)-3-hydroxybutyrate] synthesis by Cupriavidus necator DSM 545 by fed-batch cultivation on glycerol

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• 作者：Ivna Vrana 艩poljari膰 ; Markan Lopar ; Martin Koller ; Alex ; er Muhr ; Anna Salerno ; Angelika Reiterer ; Predrag Horvat
• 关键词：Cupriavidus necator ; Glycerol ; Mathematical modeling ; Optimization ; PHB
• 刊名：Journal of Biotechnology
• 出版年：December, 2013
• 年：2013
• 卷：168
• 期：4
• 页码：625-635
• 全文大小：790 K

文摘

Glycerol was utilized by Cupriavidus necator DSM 545 for production of poly-3-hydroxybutyrate (PHB) in fed-batch fermentation. Maximal specific growth rates (0.12 and 0.3 h^鈭?) and maximal specific non-growth PHB production rate (0.16 g g^鈭? h^鈭?) were determined from two experiments (inocula from exponential and stationary phase). Saturation constants for nitrogen (0.107 and 0.016 g L^鈭?), glycerol (0.05 g L^鈭?), non-growth related PHB synthesis (0.011 g L^鈭?) and nitrogen/PHB related inhibition constant (0.405 g L^鈭?), were estimated. Five relations for specific growth rate were tested using mathematical models. In silico performed optimization procedures (varied glycerol/nitrogen ratio and feeding) has resulted in a PHB content of 70.9%, shorter cultivation time (23 h) and better PHB yield (0.347 g g^鈭?). Initial concentration of biomass 16.8 g L^鈭? and glycerol concentration in broth between 3 and 5 g L^鈭? were decisive factors for increasing of productivity.