文摘
A robust recognition of phylogenetic affinities of Opalinidaeaaathe peculiar multinucleated intestine commensals of frogsaaais hindered by the absence of reliable molecular data. Up to now all attempts to sequence opalinid genes failed, as the obtained sequences labeled as Protoopalina intestinalis, Cepedea virguloidea, and Opalina ranarum in GenBank apparently originate from a zygomycete contamination. In this paper, we present the first molecular data for the family OpalinidaeaaaSSU rRNA gene of P. intestinalis. Our phylogenetic analyses undoubtedly show opalinids as a sister group to Proteromonas within the Stramenopila clade, confirming the monophyly of Pattersonaaas order Slopalinida. The enigmatic genus Blastocystis is resolved with high statistical support as a sister group to Slopalinida. The information contained in the SSU rRNA gene proved insufficient to uncover broader affinities of this group to other groups of Stramenopila. Nevertheless, our analyses clearly demonstrate that Cavalier-Smithaaas phylum Bigyra, which comprises Oomycetes and their relatives together with Slopalinida and Blastocystis, is not monophyletic.