Validation of UHPLC-MS/MS methods for the determination of kaempferol and its metabolite 4-hydroxyphenyl acetic acid, and application to in vitro blood-brain barrier and intestinal drug permeability studies
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- 作者：Fahimeh Moradi-Afrapolia ; b ; Mouhssin Oufira ; Fruzsina R. Walterc ; Maria A. Delic ; Martin Smieskod ; Volha Zabelaa ; Veronika Butterwecke ; f ; Matthias Hamburgera ; matthias.hamburger@unibas.ch" class="auth_mail" title="E-mail the corresponding author
- 关键词：4-HPAA ; 4-hydroxyphenyl acetic acid ; ACN ; acetonitrile ; BSA ; bovine serum albumin ; Cal ; calibrator ; CCl ; cell layer capacitance ; CNS ; central nervous system ; CV% ; coefficient of variation ; CPT-cAMP ; 8-(4-chlorophenylthio)-adenosine-3&prime ; 5&prime ; -cyclic monophosphate sodium salt ; DMEM ; dulbecco&rsquo ; s modified eagle&rsquo ; s medium ; EMA ; european medicines agency ; ER ; efflux ratio ; ESI ; electro-spray ionization ; FBS ; fetal bovine serum ; FDA ; food and drug administration ; hBMEC ; immortalized h
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：5 September 2016
- 年：2016
- 卷：128
- 期：Complete
- 页码：264-274
- 全文大小：1817 K
文摘
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Intestinal and BBB transport of kaempferol and 4-HPAA studied in cell-based models.
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Fully validated UHPLC–MS/MS quantification methods were developed and used.
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Kaempferol showed permeation in the BBB models, but not 4-HPAA.
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Data obtained with the human and rat BBB models showed good correlation.