RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design

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• 作者：Olle Terenius^a ; ^{olle.terenius@ekol.slu.se} ; Alexie Papanicolaou^b ; ^c ; Jennie S. Garbutt^d ; Ioannis Eleftherianos^e ; Hanneke Huvenne^f ; Sriramana Kanginakudru^g ; Merete Albrechtsen^h ; Chunju Anⁱ ; Jean-Luc Aymeric^j ; Andrea Barthel^k ; Piotr Bebas^l ; Kavita Bitra^m ; Alejandra Bravoⁿ ; Franç ; ois Chevalier^j ; Derek P. Collinge^c ; ^o ; Cristina M. Crava^p ; Ruud A. de Maagd^q ; Bernard Duvic^j ; Martin Erlandson^r ; ^s ; Ingrid Faye^t ; Gabriella Felfö ; ldi^u ; Haruhiko Fujiwara^v ; Ryo Futahashi^v ; ^w ; Archana S. Gandhe^g ; Heather S. Gatehouse^x ; Laurence N. Gatehouse^x ; Jadwiga M. Giebultowicz^y ; Isabel Gó ; mezⁿ ; Cornelis J.P. Grimmelikhuijzen^z ; Astrid T. Groot^k ; Frank Hauser^z ; David G. Heckel^k ; Dwayne D. Hegedus^r ; ^A ; Steven Hrycaj^B ; Lihua Huang^C ; J. Joe Hull^D ; ^E ; Kostas Iatrou^F ; Masatoshi Iga^f ; Michael R. Kanostⁱ ; Joanna Kotwica^l ; Changyou Li^C ; Jianghong Li^C ; Jisheng Liu^f ; Magnus Lundmark^h ; Shogo Matsumoto^D ; Martina Meyering-Vos^G ; Peter J. Millichap^d ; Antó ; nia Monteiro^H ; Nirotpal Mrinal^g ; Teruyuki Niimi^I ; Daniela Nowara^z ; Atsushi Ohnishi^D ; Vicencio Oostra^J ; Katsuhisa Ozaki^K ; Maria Papakonstantinou^F ; Aleksandar Popadic^B ; Manchikatla V. Rajam^L ; Suzanne Saenko^J ; Robert M. Simpson^x ; Mario Soberó ; nⁿ ; Michael R. Strand^m ; Shuichiro Tomita^M ; Umut Toprak^s ; ^N ; Ping Wang^C ; Choon Wei Wee^O ; Steven Whyard^P ; Wenqing Zhang^Q ; Javaregowda Nagaraju^g ; Richard H. ffrench-Constant^b ; Salvador Herrero^p ; ^q ; Karl Gordon^c ; Luc Swevers^F ; Guy Smagghe^f
• 关键词：RNA interference ; Lepidoptera ; Delivery methods ; Tissue uptake ; Gene function ; dsRNA properties
• 刊名：Journal of Insect Physiology
• 出版年：2011
• 出版时间：February 2011
• 年：2011
• 卷：57
• 期：2
• 页码：231-245
• 全文大小：665 K

文摘

Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments.