Modulation of expression of IL-8 gene in bronchial epithelial cells by 5-methoxypsoralen
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- 作者：Elena Nicolis ; Ilaria Lampronti ; Maria Cristina Dechecchi ; Monica Borgatti ; Anna Tamanini ; Valentino Bezzerri ; Nicoletta Bianchi ; Martina Mazzon ; Irene Mancini ; Maria Grazia Giri ; Paolo Rizzotti ; Robe
- 关键词：Cystic fibrosis ; Cytokine ; Inflammation ; Pseudomonas aeruginosa ; Psoralen
- 刊名：International Immunopharmacology
- 出版年：2009
- 出版时间：November 2009
- 年：2009
- 卷：9
- 期：12
- 页码：1411-1422
- 全文大小：979 K
文摘
Persistent recruitment of neutrophils in the bronchi of cystic fibrosis patients contributes to airway tissue damage, suggesting the importance of intervening on the expression of the neutrophil chemokine IL-8. Extracts from plants have been investigated to select components able to reduce IL-8 expression in bronchial epithelial cells challenged with Pseudomonas aeruginosa. Extracts and purified components have been added to cells 24 h before pro-inflammatory challenge with P. aeruginosa and IL-8 transcription was quantified in the IB3-1 CF cells in vitro. P. aeruginosa-dependent IL-8 mRNA induction was increased by Argemone mexicana and Vernonia anthelmintica whereas no significant modification of transcription was observed with Aphanamixis polystachya, Lagerstroemia speciosa and Hemidesmus indicus. Finally, inhibition of IL-8 was observed with Polyalthia longifolia (IC50 = 200 μg/ml) and Aegle marmelos (IC50 = 20 μg/ml). Compounds from A. marmelos were isolated and identified by GC–MS. No significant effect was observed with butyl-p-tolyl sulphate, whereas the inhibition obtained with 6-methyl-4-chromanone concentration was accompanied by an anti-proliferative effect. On the contrary, 5-methoxypsoralen resulted in IL-8 inhibition at 10 μM concentration, without effects on cell proliferation. In synthesis, 5-methoxypsoralen can be taken into consideration to investigate mechanisms of neutrophil chemotactic signalling and for its potential application in modulating the excessive CF lung inflammation.