Differential proteomic and phenotypic behaviour of papillary and anaplastic thyroid cell lines
详细信息 查看全文
- 作者：Rosa Musso ; Gianluca Di Cara ; Nadia Ninfa Albanese ; Maria Rita Marabeti ; Patrizia Cancemi ; D¨¨sir¨¨e Martini ; Ester Orsini ; Carla Giordano ; Ida Pucci-Minafra
- 关键词：Thyroid cancer ; Papillary ; Anaplastic ; BCPAP cells ; 8505C cells ; Proteomics
- 刊名：Journal of Proteomics
- 出版年：2013
- 出版时间：2 September, 2013
- 年：2013
- 卷：90
- 期：Complete
- 页码：115-125
- 全文大小：1535 K
文摘
Thyroid carcinomas account for a minority of all malignant tumours but, after those of the gonads, they represent the most common forms of endocrine cancers. They include several types, among which the papillary thyroid cancer (PTC) and the anaplastic thyroid cancer (ATC) are the best known. The two hystotypes display significant biological and clinical differences: PTC is a well differentiated form of tumour with a high incidence and a good prognosis, while the ATC is less frequent but represents one of the most aggressive endocrine tumours with morphological features of an undifferentiated type. To date, as far as we know, no conclusive studies, useful to design arrays of molecular markers, have been published illustrating the phenotypic and proteomic differences between these two tumours. The aim of this work was to perform a comparative analysis of two thyroid cancer cell lines, derived respectively from papillary (BCPAP) and anaplastic (8505C) thyroid carcinomas. The comparative analysis included cell behaviour assays and proteomic analysis by 2D-PAGE and mass spectrometry. The results have highlighted a new proteomic signature for the anaplastic carcinoma-derived cells, consistent with their high proliferation rate, motility propensity and metabolic shift, in relation to the well-differentiated PTC cells.
This article is part of a Special Issue entitled: From Genome to Proteome: Open Innovations.