FAS −670 A/G polymorphism may be associated with the depletion of CD4⁺ T lymphocytes in HIV-1 infection

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• 作者：Renata Bezerra Hermes ; Bá ; rbara Brasil Santana ; Sandra Souza Lima ; Rosimar Neris Martins Feitosa ; Marluí ; sa de Oliveira Guimarã ; es Ishak ; Ricardo Ishak ; Antonio Carlos Rosá ; rio Vallinoto ; ^{vallinoto@ufpa.br" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ACRS ; amplification-created restriction site ; ARVT ; anti-retroviral therapy ; bDNA ; branched DNA ; FASL ; FAS ligand ; PBMC ; peripheral blood mononuclear cells ; PCR ; polymerase chain reaction ; RFLP ; restriction fragment length polymorphism
• 刊名：Human Immunology
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：76
• 期：10
• 页码：742-746
• 全文大小：377 K

文摘

In this study, the polymorphisms in the FAS and FASL genes was investigated in a sample of 198 HIV-1-seropositive individuals and 191 seronegative controls to evaluate a possible association between polymorphisms and the infection. The identification of the A and G alleles of the FAS −670 polymorphism was accomplished through polymerase chain reaction assays followed by digestion with the restriction enzyme MvaI. The identification of the A and G alleles of the FAS −124 polymorphism and the T and delT alleles of the FAS −169 polymorphism were performed using the amplification-created restriction site method followed by restriction fragment length polymorphism reactions. The comparative analysis of allelic and genotypic frequencies between the groups did not reveal any significant differences. However, the quantitative analysis of CD4⁺ T lymphocytes suggests that the G allele of the FAS −670 A/G polymorphism can be a protective factor against the depletion of these cells in the course of an HIV-1 infection. Polymorphisms in the FAS and FASL genes were not associated with the number of CD8⁺ T lymphocytes or the plasma viral load. Our findings suggest that the FAS −670 polymorphism may be associated with apoptosis of CD4⁺ T lymphocytes after infection by HIV-1.