Kinetic study of a novel thermo-stable ¦Á-carbonic anhydrase for biomimetic CO2 capture
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- 作者：Maria Elena Russo ; Giuseppe Olivieri ; Clemente Capasso ; Viviana De Luca ; Antonio Marzocchella ; Piero Salatino ; Mos¨¨ Rossi
- 关键词：CO2 capture ; Carbonic anhydrase ; Absorption ; Thermo-stable enzyme
- 刊名：Enzyme and Microbial Technology
- 出版年：2013
- 出版时间：10 September, 2013
- 年：2013
- 卷：53
- 期：4
- 页码：271-277
- 全文大小：942 K
文摘
Biomimetic CO2 capture includes environmentally friendly solutions based on carbonic anhydrase (CA), an enzyme that increases CO2 absorption rate in conventional acid-gas scrubbing processes. The present contribution reports the characterization of a new recombinant carbonic anhydrase, SspCA, isolated from the thermophile bacterium Sulphurhydrogenibium yellowstonense sp. YO3AOP1.
The kinetics of SspCA was characterized in terms of first order CO2 hydration rate according to a procedure based on CO2 absorption tests in a stirred cell apparatus. The first order kinetic constant at 25 ¡ãC was 9.16 ¡Á 106 L/(mol s).
An appropriate investigation on SspCA stability was carried out to assess its long-term resistance to high temperatures as in all capture processes based on absorption/vacuum-desorption cycles. Its half-life was 53 and 8 days at 40 ¡ãC and 70 ¡ãC, respectively.