Spatiotemporal dynamics of epileptiform propagations: Imaging of human brain slices

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• 作者：Hiroki Kitaura^a ; ^{kitaura@bri.niigata-u.ac.jp" rel="nofollow} ; Tetsuya Hiraishi^b ; Hiroatsu Murakami^c ; Hiroshi Masuda^c ; Masafumi Fukuda^b ; Makoto Oishi^b ; Masae Ryufuku^a ; Yong-Juan Fu^a ; Hitoshi Takahashi^a ; Shigeki Kameyama^c ; Yukihiko Fujii^b ; Katsuei Shibuki^d ; Akiyoshi Kakita^a
• 关键词：Epilepsy ; Cerebral cortex ; Flavoprotein fluorescence ; Propagation ; Human
• 刊名：NeuroImage
• 出版年：2011
• 出版时间：1 September 2011
• 年：2011
• 卷：58
• 期：1
• 页码：50-59
• 全文大小：2036 K
• ISSN：S1053811911005544

文摘

Seizure activities often originate from a localized region of the cerebral cortex and spread across large areas of the brain. The properties of these spreading abnormal discharges may account for clinical phenotypes in epilepsy patients, although the manner of their propagation and the underlying mechanisms are not well understood. In the present study we performed flavoprotein fluorescence imaging of cortical brain slices surgically resected from patients with partial epilepsy caused by various symptomatic lesions. Elicited neural activities in the epileptogenic tissue spread horizontally over the cortex momentarily, but those in control tissue taken from patients with brain tumors who had no history of epilepsy demonstrated only localized responses. Characteristically, the epileptiform propagation comprised early and late phases. When the stimulus intensity was changed gradually, the early phase showed an all-or-none behavior, whereas the late phase showed a gradual increase in the response. Moreover, the two phases were propagated through different cortical layers, suggesting that they are derived from distinct neural circuits. Morphological investigation revealed the presence of hypertrophic neurons and loss of dendritic spines, which might participate in the aberrant activities observed by flavoprotein fluorescence imaging. These findings indicate that synchronized activities of the early phase may play a key role in spreading abnormal discharges in human cortical epilepsies.