Efficient in vivo depletion of CD8⁺ T lymphocytes in common marmosets by novel CD8 monoclonal antibody administration

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• 作者：Tomoyuki Yoshida ; Saori Suzuki ; Yuki Iwasaki ; Akihisa Kaneko ; Akatsuki Saito ; Yuki Enomoto ; Atsunori Higashino ; Akino Watanabe ; Juri Suzuki ; Kenichi Inoue ; Teiko Kuroda ; Masahiko Takada ; Ryoji Ito ; Mamoru Ito ; Hirofumi Akari
• 关键词：Monoclonal antibody ; CD8 T lymphocyte ; Common marmoset ; In vivo depletion
• 刊名：Immunology Letters
• 出版年：2013
• 出版时间：July-August, 2013
• 年：2013
• 卷：154
• 期：1-2
• 页码：12-17
• 全文大小：1659 K

文摘

In order to directly demonstrate the roles of CD8⁺ T lymphocytes in non-human primates, in vivo depletion of the CD8⁺ T cells by administration of a CD8-specific monoclonal antibody (mAb) is one of the crucial techniques. Recently, the common marmoset (Callithrix jacchus), which is classified as a New World monkey, has been shown useful as an experimental animal model for various human diseases such as multiple sclerosis, Parkinson's disease and a number of infectious diseases. Here we show that an anti-marmoset CD8 mAb 6F10, which we have recently established, efficiently depletes the marmoset CD8⁺ T lymphocytes in vivo, i.e., the administration of 6F10 induces drastic and specific reduction in the ratio of the CD8⁺ T cell subset for at least three weeks or longer. Our finding will help understand the pivotal role of CD8⁺ T cells in vivo in the control of human diseases.