Lipopolysaccharide-induced multinuclear cells: Increased internalization of polystyrene beads and possible signals for cell fusion

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• 作者：Mayumi Nakanishi-Matsui ; Shio Yano ; Masamitsu Futai
• 关键词：LPS ; lipopolysaccharide ; TLR4 ; toll-like receptor-4 ; RANKL ; receptor activator of nuclear factor 魏B ligand ; RANK ; receptor activator of nuclear factor 魏B ; FBGC ; foreign body giant cell ; MEM魏 ; minimum essential medium alpha medium ; PBS ; phosphate-buffered saline ; TRAP ; tartrate-resistant acid phosphatase ; FITC ; fluorescein isothiocyanate ; IL6 ; interleukin 6 ; IL10 ; interleukin 10 ; TNF伪 ; tumor necrosis factor 伪 ; ELISA ; enzyme-linked immunosorbent assay ; MAPK ; mitogen-activated protein kinase
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：1 November, 2013
• 年：2013
• 卷：440
• 期：4
• 页码：611-616
• 全文大小：1750 K

文摘

A murine macrophage-derived line, RAW264.7, becomes multinuclear on stimulation with lipopolysaccharide (LPS), an outer membrane component of Gram-negative bacteria. These multinuclear cells internalized more polystyrene beads than mononuclear cells or osteoclasts (Nakanishi-Matsui, M., Yano, S., Matsumoto, N., and Futai, M., 2012). In this study, we analyzed the time courses of cell fusion in the presence of large beads. They were internalized into cells actively fusing to become multinuclear. However, the multinuclear cells once formed showed only low phagocytosis activity. These results suggest that formation of the multinuclear cells and bead internalization took place simultaneously. The formation of multinuclear cells was blocked by inhibitors for phosphoinositide 3-kinase, phospholipase C, calcineurin, and c-Jun N-terminal kinase. In addition, interleukin 6 and 10 also exhibited inhibitory effects. These signaling molecules and cytokines may play a crucial role in the LPS-induced multinuclear cell formation.