Sequential analysis of RNA synthesis by microchip electrophoresis

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• 作者：Yoshihiro Umemoto ; Masatoshi Kataoka ; Shouki Yatsushiro ; Masahiro Watanabe ; Jun-ichi Kido ; Rei Kakuhata ; Takenori Yamamoto ; Yasuo Shinohara ; Yoshinobu Baba
• 刊名：Analytical Biochemistry
• 出版年：2009
• 出版时间：1 May 2009
• 年：2009
• 卷：388
• 期：1
• 页码：161-163
• 全文大小：190 K

文摘

We describe the potential of microchip electrophoresis with a Hitachi SV1100, which can be used to evaluate the integrity of total RNA, for the analysis of synthesized RNA. There was little interference by DNA and/or the components of the in vitro transcription system with the microchip electrophoresis. The fluorescence intensity corresponding to the synthesized RNA increased in a time-dependent manner as to the RNA synthesis reaction on sequential analysis. A result can be obtained in 160 s and only 1/10 aliquots of samples, compared with the conventional method, are required. These results indicate the potential of microchip electrophoresis for sequential analysis of RNA synthesis.