Antigen-capture ELISA for the detection of Rift Valley fever virus nucleoprotein using new monoclonal antibodies

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• 作者：Shuetsu Fukushi ; Mina Nakauchi ; Tetsuya Mizutani ; Masayuki Saijo ; Ichiro Kurane ; Shigeru Morikawa ; ^{morikawa@nih.go.jp}
• 关键词：Rift Valley fever virus ; Monoclonal antibody ; Antigen-capture ELISA
• 刊名：Journal of Virological Methods
• 出版年：2012
• 出版时间：March 2012
• 年：2012
• 卷：180
• 期：1-2
• 页码：68-74
• 全文大小：1300 K

文摘

Monoclonal antibodies (MAbs) raised against the nucleoprotein (NP) of Rift Valley fever virus (RVFV) were developed, and an antigen-capture enzyme-linked immunosorbent assay (Ag-capture ELISA) system was developed for the detection of RVFV NP. The assay detected RVFV antigen from culture supernatants containing as little as 7.8-31.3 pfu per 100 ¦Ìl. Reactivity with various truncated NPs indicated that MAb C10-54 bound only to the full-length NP, probably due to recognition of a conformational epitope, whereas MAbs G2-36 and D5-59 bound to a linear epitope ranging from amino acid residues 195-201 in the C-terminal region. Based on the alignments of the amino acid sequence of RVFV NP, the epitope regions of MAbs G2-36 and D5-59 were completely conserved among all RVFV strains. These results suggest that the MAbs are applicable to the Ag-capture ELISA for the diagnosis of RVFV infections.