Suppressive effect of hot water extract of wasabi (Wasabia japonica

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• 作者：Tetsuro Ogawa ; Hiromasa Tabata ; Takuya Katsube ; Yukari Ohta ; Yukikazu Yamasaki ; Masayuki Yamasaki ; Kuninori Shiwaku
• 关键词：Wasabi ; 3T3-L1 cell ; Adipocyte ; Differentiation ; PPARγ ; C/EBPα
• 刊名：Food Chemistry
• 出版年：2010
• 出版时间：15 January 2010
• 年：2010
• 卷：118
• 期：2
• 页码：239-244
• 全文大小：319 K

文摘

This study investigated the effect of hot water extract of wasabi (Wasabia japonica Matsum.) leaves (WLE), without its specific pungent constituents, such as allyl isothiocyanate, on the differentiation of 3T3-L1 preadipocytes. WLE suppressed the increase in glycerol-3-phosphate dehydrogenase (GPDH) activity and triglyceride (TG) accumulation, markers of adipogenesis, in a dose-dependent manner. Quantitative real time RT-PCR results showed that WLE significantly reduced the mRNA expression levels of peroxisome proliferator-activated receptor (PPAR) γ and CCAAT/enhancer-binding protein (C/EBP) α, both key adipogenic transcription factors, as subsequently were the mRNA expression levels of their target genes, such as adipocyte fatty acid binding protein 2 (aP2). Western blot analysis results showed that the protein expression levels of both PPARγ and C/EBPα were also inhibited by WLE. Thus, WLE suppressed the differentiation of 3T3-L1 preadipocytes, and the suppressive effect was mediated, in part, through the altered regulation of PPARγ, C/EBPα, and other specific genes, such as aP2. These results suggest that WLE may prevent obesity and insulin resistance by inhibiting the differentiation of preadipocytes.