A dynein light chain of sea urchin sperm flagella is a homolog of mouse Tctex 1, which is encoded by a gene of the t complex sterility locus

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• 作者：Kagami ; Osamu ; Gotoh ; Masuo ; Makino ; Yumiko ; Mohri ; Hideo ; Kamiya ; Ritsu ; Ogawa ; Kazuo
• 关键词：cDNA ; Meiotic drive ; Transmission ratio distortion ; Cytoplasmic dynein ; cDNA=DNA complementary to RNA ; DHC=dynein heavy chain ; IC=intermediate chain ; LC=light chain ; SDS&ndash ; PAGE=SDS polyacrylamide gel electrophoresis ; TRD=transmission ratio distortion
• 刊名：Gene
• 出版年：1998
• 出版时间：May 12, 1998
• 年：1998
• 卷：211
• 期：2
• 页码：383-386
• 全文大小：204 K

文摘

The outer-arm dynein of sea urchin sperm flagella contains six light chains with molecular masses of 23.2, 20.8, 12.3, 11.5, 10.4 and 9.3 kDa. We have cloned a cDNA for the 12.3 kDa polypeptide (light chain 3) and found that this protein is highly homologous to mouse Tctex1, a protein encoded by a member of the multigene family in the t complex region that is involved in male sterility and the development of the germ cells. Tctex1 has recently been shown to be homologous to a light chain of cytoplasmic dynein. Therefore, the cytoplasmic dynein light chain has been implicated in the mechanism for the transmission ratio distortion (meiotic drive) that is characteristic of t haplotypes in mice. Our present finding, however, indicates that axonemal light chain 3 must be considered equally important.