In transient transfection experiments using a reporter plasmid carrying a luciferase gene under control of HCMV major immediate-early enhancer/promoter (MIEP), the statins had no effect on the MIEP activity in HEL cells. In U373-IE1 cells established by transfection with the IE1 gene driven by the MIEP into astrocytoma U373-MG cells, which are permissive for HCMV infection, the presence of statins did not affect IE1 protein expression. To investigate the difference in results between virus-infected and gene-transfected cells, we used UV-irradiated HCMV, which is capable only of binding to and internalizing in the cells. The experiments with UV-irradiated HCMV demonstrated that statins decreased IE1 protein expression in U373-IE1 cells infected with UV-inactivated virus.
Virus-host responses triggered by UV-irradiated HCMV particle binding and penetration, occurring at an early stage of infection, might contribute to the inhibition of IE1 protein expression by statins.