Ectopic calcification: importance of common nanoparticle scaffolds containing oxidized acidic lipids
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- 作者：Hiromi Kumon ; Eiji Matsuura ; Noriyuki Nagaoka ; Toshio Yamamoto ; Shinya Uehara ; Motoo Araki ; Yukana Matsunami ; Kazuko Kobayashi ; Akira Matsumoto
- 关键词：Nanoparticles ; Nanobacteria-like particles ; Ectopic calcification ; Oxidized lipids
- 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
- 出版年：February, 2014
- 年：2014
- 卷：10
- 期：2
- 页码：441-450
- 全文大小：3184 K
文摘
The term nanobacteria, sometimes referred to as nanobacteria-like particles (NLPs), is presently recognized as a misnomer for inert calcified nanoparticles. However, misinterpretation of its propagation as a living organism still continues. Ultrastructural and elemental analyses, combining immuno-electron microscopy with an original NLP isolate (P-17) derived from urinary stones, and an IgM monoclonal antibody (CL-15) raised against P-17 have now revealed that, oxidized lipids with acidified functional groups were key elements in NLP propagation. Lamellar structures composed of acidic/oxidized lipids provided structural scaffolds for carbonate apatite crystals. During in vitro culture, lipid peroxidation induced by 纬-irradiation of FBS was a major cause of accelerated NLP propagation. In pathological tissue samples from hyperlipidemic atherosclerosis-prone mice, CL-15 co-localized with fatty plaques, macrophage infiltrates and osteocalcin staining of aortic valve lesions. These observations indicate that naturally occurring NLP composed of mineralo-oxidized lipids complexes are generated as by-products rather than etiological agents of chronic inflammation.
From the Clinical Editor
The term 鈥渘anobacteria-like particles (NLPs)鈥?is presently recognized as a misnomer for inert calcified nanoparticles as opposed to living organisms. This study convincingly demonstrates that naturally occurring NLPs composed of mineralo-oxidized lipid complexes are generated as by-products rather than etiological agents of chronic inflammation.